Thiamethoxam Colloidal Gold Quick Detection Card Instruction Manual

Product Number: YP084C02K

1 Principle and Use

This product applies the principle of competitive inhibition colloidal gold immunochromatography to detect thiamethoxam residues in vegetables and fruits. After the sample solution is dropped into the sample hole of the detection card, the thiamethoxam in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the thiamethoxam conjugate on the cellulose film. The detection result is judged by the color depth of C and T lines.

2 technical indicators, the minimum detection limit: 10 μg/kg (ppb)

3 You need to bring your own tools

electronic balance (0.1-100 grams), scissors, forceps, disposable cups, vortex mixer, pipette (0.1-1 ml), timer.

4 sample pretreatment 11727798400.1 The sample needs to be restored to room temperature (20-30 ° C) before testing.

4 Take an appropriate amount of sample, cut to about 1 cm square, weigh 1 gram of chopped sample in a 20 mL graduated sample cup, add 1 mL of dilution, manually shake and mix (about 50 times/minute) 2 minutes, let stand for 1 minute, as the sample solution;

4 Take a 1.5 mL centrifuge tube, dilute the sample solution with the dilution in different proportions according to the following table, the diluted solution is the liquid to be tested;

According to different residue limit requirements, the above sample treatment solution is treated according to the following scheme:

Detection limit

Sample treatment solution

Thiamethoxam dilution

Corn (100 ppb)

120 μL

0 μL

Cabbage, potato, loofah (200 ppb)

100 μL

100 μL

Cowpea, ginger (300 ppb)

100 μL

200 μL

nightshade vegetables, peppers (1 ppm)

100 μL

900 μL

eggplant (500 ppb)

100 μL

400 μL

leafy vegetables (3 ppm)

50 μL

1450 μL

dried peppers (7 ppm)

20 μL

1380 μL

5 sample testing

5 Tear the test card aluminum foil packaging bag, remove the test card and dropper, and place it on a flat, clean countertop.

5.2 Pipette or dropper 120 μL (about 4 drops) of the above liquid to be tested in the sample hole (S) of the test card;

5.3 Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, and the interpretation is invalid at other times.

6 the result judges

negative, positive, invalid 117277984

Positive (+): The purplish-red line appears on the C line, and the T line does not develop color, indicating that the concentration of thiamethoxam in the sample is higher than the detection limit.

Invalid: In the detection window, the control line (C) does not appear purplish-red line.

7 Precautions

7 Products that have expired or the aluminum foil bag is damaged should not be used.

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid failure.

7 Do not touch the white film surface in the center of the test card.

7 The liquid extraction dropper should not be mixed to avoid cross-contamination.

7.5 The sample solution to be tested should be clear, free of cloudy particles, and free of bacterial contamination, otherwise it will easily lead to abnormal color development and other phenomena, which will affect the result

8 Storage and shelf life

8 Storage conditions: 4-30 ℃ protected from light, do not freeze.

8.2 Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (including dropper, desiccant)

10 parts

20 parts

thiamethoxam dilution

1 bottle

2 bottle

20 mL graduated sample cup

1 pieces

2 pieces

1 pieces

1 mL centrifuge tube

10 pieces

20 pieces

manual

1 parts

1 parts