Fenthion Colloidal Gold Quick Detection Card Instruction Manual

Product Number: YB102C01K

1 Principle and Use

This product is made of the principle of competitive inhibition colloidal gold immunochromatography for detecting fenthion residues. After the sample solution is dropped into the sample well of the detection card, the fenthion in the sample solution binds to the gold standard antibody, thereby preventing the gold standard antibody from binding to the fenthion conjugate on the cellulose membrane. The detection result is judged by the color depth of C and T lines.

2 Technical Indicators

Detection Sensitivity: 50 μg/kg (ppb)

3 Tools to be provided

Electronic balance (0.1-100 grams), scissors, disposable cups, forceps, vortex mixer, pipette (0.1-1 mL), timer.

4 sample pretreatment

4 Take the sample epidermis, cut into fragments of about 1 cm;

4 Weigh 2 grams of shredded sample in a 15 mL centrifuge tube with a balance; 117277984000.3 Add 3 mL of fenthion dilution, fully oscillate for 2 minutes, let stand for 1 minute, to be inspected;

5 sample detection

5.1 Tear open the aluminum foil packaging bag of the detection card, take out the detection card/gold label microporous/dropper (a total of 3 kinds of consumables), and put it on a flat and clean countertop.

5 with a pipette or aluminum foil bag matching dropper to absorb the above liquid to be tested 120 μL (about 4 drops) into the gold standard micropores, the red substance in the micropores and the solution to be tested are fully mixed with the dropper, let stand for 2 minutes, and then mix;

5 All the liquid in the gold standard micropores is added dropwise to the sample well (S) of the detection card;

5 Start timing after adding the sample, and leave it at room temperature for 8-10 minutes to judge the result, and the interpretation is invalid at other times.

6 result judgment

Negative (-): T-line color development is deeper than C-line or there is no significant difference with C-line color development, indicating that there is no substance to be tested in the sample or its concentration is lower than the detection limit.

Positive (+): The color development of the T-line is significantly weaker than that of the C-line or the T-line does not develop color, indicating that the concentration of the test object in the sample is equal to or higher than the detection limit.

Invalid: The C-line does not appear, indicating that the operation process is incorrect or the test card has failed. In this case, the instructions should be carefully read again and re-tested with a new test card.

7 precautions

7 Products that have expired or the aluminum foil bag is damaged should not be used.

7 When the test card is taken out of the refrigerator, it should be restored to room temperature and opened. The opened test card should be used as soon as possible to avoid moisture.

7 Do not touch the white film surface in the center of the test card.

7.4 The liquid extraction dropper should not be mixed to avoid cross contamination.

7 The sample solution to be tested should be clear, no cloudy particles, and no bacterial contamination, otherwise it will easily lead to abnormal phenomena such as blockage and inconspicuous color development, which will affect the judgment of experimental results.

7 If you need to directly test the standard product, you need to use the fenthion dilution in the kit.

8 Storage and shelf life

8 Storage conditions: 4-30 ° C Store in the dark, do not freeze.

8.2 Shelf life: valid period 1 year, see the outer packaging for the production date.

9 kit composition

specification

composition

10 times/box

20 times/box

test card (containing gold standard micropores, dropper, desiccant)

10 parts

20 parts

fenthion dilution

1 bottle

2

15 mL centrifuge tube

1 pieces

2 pieces

1 mL straw

10 pieces

20 pieces

manual

1 parts

1 parts